Supplemental Figure S7.

Antibody neutralization of heparin-binding EGF-like growth factor (HBEGF) restores forkhead box protein A2 (FOXA2) to attenuate mucin expression during exposure to heat-killed (HK) Blastomyces dermatitidis. These are replicates of Western blots shown in Figure 10B. BACA cells were pretreated with anti-HBEGF antibody (1 μg) or an irrelevant control mouse IgG (Ctrl IgG; 1 μg) for 2 hours before 24 hours of exposure to heat-killed B. dermatitidis (multiplicity of infection 5:1). Control (-) cells were exposed to the same volume of sterile phosphate-buffered saline. Cytoplasmic and nuclear protein extracts were separated on acrylamide-agarose gels (mucins) and normal 10% acrylamide gels [FOXA2, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and H3], and probed with specific antibodies against each protein. GAPDH and H3 were used as loading controls.