Figure 4.

Histoplasma capsulatum induces mucin expression in the immortalized canine airway carcinoma (BACA) cells. BACA cells at 90% confluency were challenged with live (LV) or heat-killed (HK) H. capsulatum strain G217B at the indicated multiplicity of infection (MOI). Control (Ctrl) cells were exposed to the same volume of sterile phosphate-buffered saline. Cytoplasmic and nuclear protein extracts were separated on acrylamide-agarose (mucins) and acrylamide [forkhead box protein A2 (FOXA2), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and H3] gels, respectively, and probed with specific antibodies against each protein. GAPDH and H3 were used as loading controls. The experiments were independently performed three times with similar results. Western blots from one typical experiment are shown.