Reagent | Final concentration | Amount |
---|---|---|
1 M sodium acetate | 55 mM | 2.75 mL |
1 M potassium acetate | 40 mM | 2 mL |
100 mM magnesium chloride | 1.2 mM | 0.6 mL |
100 mM calcium chloride | 1 mM | 0.5 mL |
Sucrose | 110 mM | 1.88 g |
HEPES | 100 mM | 1.19 g |
ddH2O | n/a | Up to 50 mL |
Total | n/a | 50 mL |