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. 2020 Dec 30;2(1):100248. doi: 10.1016/j.xpro.2020.100248

Figure 1.

Figure 1

Scheme for ROS-low LSCs and ROS-high AML blasts cell sorting and expected results

(A) Flow scheme demonstrating ROS sorting to enrich ROS-low LSCs and AML blasts. Step 1 is FSC versus SSC which is used to isolate cells. Step 2 is FSC-A versus FSC-H used to isolate single cells. Step 3 is FSC versus DAPI which is used to isolated DAPI- live cells. Step 4 is CD19 versus CD3 used to exclude lymphocytes (CD3-CD19-). Step 5 is CD45 versus SSC used to isolate AML blasts. Step 6 is CellROX staining, CellROX deep red shown here.

(B) Example of the number of colonies formed from ROS-low and ROS-high cells. Data adapted from Jones et al. (2018).

(C) Example of engraftment into NSGS mice from ROS-low and ROS-high cells. Data from Pei et al. (2018).