REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-human CD45 BB515 (HI30)	BD	Cat#564585; RRID: AB_2732068
Mouse anti-human CD3 PE-Cy7 (SP34-2)	BD	Cat#557749; AB_396855
Mouse anti-human CD19 PE (HIB19)	BD	Cat#555413; RRID: AB_395813
OKT3	Sigma-Aldrich	86022706-DNA-5UG
Biological samples
Primary AML specimens	University of Colorado Hematologic Malignancies Tissue bank	N/A
Chemicals, peptides, and recombinant proteins
IMDM	Thermo Fisher	12440-053
FBS	Corning	35077CV
Pen/strep	thermosphere	15140122
Serum-free metabolomics media	Invitra	Custom https://invitria.com/custom-media-formulation/
2-Mercaptoethanol	Sigma-Aldrich	M3148-100ML
Low-density lipoprotein	Sigma-Aldrich	437644-10MG
PBS	Corning	21040CV
SCF	PeptroTech	300-07-100ug
FLT3	PeptroTech	300-19-100ug
IL3	PeptroTech	200-03-100ug
DNase I	Sigma-Aldrich	4536282001
DAPI	Sigma-Aldrich	D9542-5MG
CellRox deep red	thermosphere	C10422
Human methylcellulose	R&D Systems	HSC005
Busulfan	Sigma-Aldrich	B2635-25G
NaCl	Sigma-Aldrich	S7653-250G
Experimental models: organisms/strains
NOD.Cg-PrkdcscidIl2rgtm1Wjl Tg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ (NSGS) mice	The Jackson Laboratory	Cat# JAX:013062, RRID: IMSR_JAX:013062
Software and algorithms
FlowJo v10	FlowJo LLC	https://www.flowjo.com