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. 2020 Dec 23;8:594785. doi: 10.3389/fcell.2020.594785

FIGURE 2.

FIGURE 2

CircHmbox1 is involved in TNF-α-induced osteoclasts differentiation. (A) TRAP staining was showed in osteoclasts silencing or overexpressing circHmbox1 under RANKL and M-CSF for 7 days. (B) Summarized data showed that silencing of circHmbox1 significantly increased osteoclasts differentiation. However, circHmbox1 significantly inhibited Rankl-induced osteoclastogenesis. n = 6, *P < 0.05, **P < 0.01. (C) The expressions of osteoclasts formation specific genes, TRAP, ctsk and NFATc1, were analyzed by qRT-PCR in osteoclasts silencing or overexpressing circHmbox1 under RANKL and M-CSF for 7 days. n = 4, **P < 0.01. (D,E) TRAP staining showed that silencing of circHmbox1 could further enhance the promoting effect of TNF-α on osteoclast differentiation. However, overexpressing of circHmbox1 could attenuate TNF-α-induced osteoclast differentiation. n = 6, *P < 0.05. (F) The expressions of osteoclasts formation specific genes, TRAP, ctsk and NFATc1, were analyzed by qRT-PCR in osteoclasts overexpressing or silencing circHmbox1 under RANKL, M-CSF and TNF-α for 7 days. n = 4, *P < 0.05, **P < 0.01.