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. 2020 Dec 23;8:594785. doi: 10.3389/fcell.2020.594785

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Copyright © 2020 Liu, Li, Huang, Hu, Jiang, Xu, Li, Deng, Ye and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MiR-1247-5p targeted by circHmbox1 is involved in TNF-α-induced osteoclasts differentiation. (A) TRAP staining was showed in osteoclasts transfected with circHmbox1/si-circHmbox1 and miR-1247-5p/AMO-1247 in the presence of RANKL and M-CSF for 5 days. (B) Summarized data showed that miR-1247-5p attenuated circHmbox1-inhibited osteoclasts differentiation. However, promoting of osteoclasts differentiation by si-circHmbox1 was alleviated by AMO-1247-5p. n = 6, **P < 0.01. (C–E) The expressions of TRAP (C), NFATc1(D) and ctsk (E) were analyzed by qRT-PCR in osteoclasts transfected with circHmbox1/si-circHmbox1 and miR-1247-5p/AMO-1247 in the presence of RANKL and M-CSF for 5 days. n = 3, **P < 0.01. (F) TRAP staining was showed in osteoclasts transfected with circHmbox1/si-circHmbox1 and miR-1247-5p/AMO-1247 in the presence of RANKL, M-CSF and TNF-α for 5 days. (G) Summarized data showed that silencing of circHmbox1 could enhance TNF-α-induced osteoclast differentiation, which could be reduced by AMO-1247-5p. MiR-1247-5p also weakened the inhibitory effect of circHmbox1 on TNF-α-induced osteoclast differentiation. n = 6, *P < 0.05, **P < 0.01. (H,I) The expressions of TRAP (H) and NFATc1(I) were analyzed by qRT-PCR in osteoclasts transfected with circHmbox1/si-circHmbox1 and miR-1247-5p/AMO-1247 in the presence of RANKL, M-CSF and TNF-α for 5 days. n = 3, **P < 0.01.