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. 2020 Dec 23;8:594785. doi: 10.3389/fcell.2020.594785

FIGURE 6.

FIGURE 6

Exosomal with low circHmbox1 expression from osteoclasts treated with TNF-α inhibits osteoblast differentiation primarily through targeting miR-1247-5p. (A) The expressions of miR-1247-5p were analyzed by qRT-PCR in osteoblasts co-cultured with Raw264.7 cells transfected with circHmbox1/si-circHmbox1 in the presence of RANKL and TNF-α. n = 3, **P < 0.01. (B) The expressions of miR-1247-5p were analyzed by qRT-PCR in osteoblasts transfected with circHmbox1/si-circHmbox1. n = 3, **P < 0.01. (C,D) The expressions of Runx2 (C) and Osterix (D) were analyzed by qRT-PCR in osteoblasts co-cultured with Raw264.7 cells transfected with circHmbox1/si-circHmbox1 in the presence of RANKL and TNF-α. n = 3, **P < 0.01. (E,F): ALP staining (E) and AR staining (F) were observed in osteoblasts co-cultured with Raw264.7 cells transfected with circHmbox1/si-circHmbox1 in the presence of RANKL and TNF-α. n = 3.