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. 2020 Dec 23;8:593653. doi: 10.3389/fcell.2020.593653

FIGURE 2.

FIGURE 2

Knockdown of PECAM1 or CXCR4 alleviates pulpitis in mice. (A) Expression of PECAM1 and CXCR4 in dental pulp tissues determined by RT-qPCR. (B) a diagram presentation of rat treatment: after 1 week of acclimatization, shRNAs of PECAM1 and CXCR1 were injected into the left-side dental pulp tissues of rats. After 48 h, a rat model with pulpitis was induced using LPS. The serum samples were collected 24 h later to examine the expression of inflammatory cytokines in serum. Next, the rats were euthanized through intraperitoneal injection of 150 mg/kg pentobarbital sodium, and then the dental pulp tissues were collected for further use. (C) mRNA expression of PECAM1 and CXCR4 in rat dental pulp tissues examined by RT-qPCR. (D–G) expression of inflammatory cytokines TNF-α, IL-6, IL-8, and VEGF in rat serum determined by ELISA kits. (H) Pathological changes in rat dental pulp tissues observed by HE staining. (I,J) Expression of B-cell-specific protein markers CD19 and CD22 in rat dental pulp tissues determined by IHC staining. In each group, n = 6. Data were collected from three individual experiments and expressed as mean ± SD. Data were analyzed by one-way (D–J) or two-way (A,C) ANOVA followed by Tukey’s multiple comparison test. **p < 0.01 vs. sham group; ##p < 0.01 vs pulpitis + sh-NC group.