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. 2020 Dec 23;8:593653. doi: 10.3389/fcell.2020.593653

FIGURE 5.

FIGURE 5

MEF2C promotes transcription activity of PECAM1 and CXCR4. (A,B) Binding relationships between MEF2C and the promoter of PECAM1 and CXCR4 predicted on JASPAR. (C,D) Binding relationships between MEF2C and PECAM1 and CXCR4 validated through luciferase reporter gene assays. (E,F) Binding relationships between MEF2C and PECAM1 and CXCR4 validated through ChIP-qPCR assays. (G,H) Overexpressing vector of MEF2C was administrated in HDPFs, and the mRNA (G) and protein (H) expression of PECAM1 and CXCR4. I, protein levels of MEF2C, PECAM1, and CXCR4 in rat dental pulp tissues examined by western blot analysis. (J) Ccorrelation between the protein expression of MEF2C and PECAM1 and CXCR4 in rat dental pulp tissues. Data were collected from three individual experiments and expressed as mean ± SD. Data were analyzed by one-way (C,D,G, and H) or two-way ANOVA (E,F, and I) followed by Tukey’s multiple comparison test. *vs. 0 μg of MEF2C vector, anti-IgG or sham group, **p < 0.01, ***p < 0.001.