TABLE 1.
Gene | Primer sequence (5′–3′) |
PECAM1 | F: AAGTGGAGTCCAGCCGCATATC |
R: ATGGAGCAGGACAGGTTCAGTC | |
CXCR4 | F: CTCCTCTTTGTCATCACGCTTCC |
R: GGATGAGGACACTGCTGTAGAG | |
MEF2C | F: TCCACCAGGCAGCAAGAATACG |
R: GGAGTTGCTACGGAAACCACTG | |
PGE2 | F: CCTTCAAGGTTCTGTGCTCAGC |
R: CATCAGCTTAGCTGGACACTGC | |
TNF-α | F: GGTGCCTATGTCTCAGCCTCTT |
R: GCCATAGAACTGATGAGAGGGAG | |
IL-6 | F: AGACAGCCACTCACCTCTTCAG |
R: TTCTGCCAGTGCCTCTTTGCTG | |
IL-8 | F: CCATTCCGTTCTGGTACAGTCTG |
R: TTTCCGCTTCCTGAGGCTGGAT | |
GAPDH | F: GTCTCCTCTGACTTCAACAGCG |
R: ACCACCCTGTTGCTGTAGCCAA |
RT-qPCR, reverse transcription-quantitative polymerase chain reaction; PECAM1, platelet endothelial cell adhesion molecule-1; CXCR4, chemokine receptor 4; MEF2C, myocyte-enhancer factor 2C; PGE2, prostaglandin E2; TNF-α, tumor necrosis factor-α; IL-6, interleukin-6; and GAPDH, glyceraldehyde-3-phosphate dehydrogenase.