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. 2020 Oct 8;104(1):117–129. doi: 10.1093/biolre/ioaa186

Figure 1.

Figure 1

Confirmation of NEDL2 antibody specificity. (A) WB detection of recombinant NEDL2 protein in NEDL2 expression vector lysate (lanes “1”) and empty vector lysate (lanes “2”); cDNA based, predicted molecular weight of NEDL2 protein is 175.7 kDa. Comparable amounts of total vector’s endogenous proteins were loaded per lane, as shown by CBB staining. (B) Alternative validation by immune-saturation approach; WB detection of NEDL2 in MII oocytes (lanes “3”) and spermatozoa (lanes “4”) with NEDL2 antibodies saturated with NEDL2 expression vector lysate or empty vector lysate prior to incubation. Comparable amounts of total vector’s endogenous proteins were used for immunosaturation. Extracts from 100 million spermatozoa and 150 MII oocytes were loaded per lanes “3” and “4,” respectively. The blots in (B) represent the same exposure time.