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Assessment of NEDL2 content (labeling intensity) in cumulus oophorus cells before and after 40 h of in vitro maturation with oocytes, by ICC (A) and WB analysis (B), NEDL2 is detected at 91 kDa under reducing conditions and the lower mass forms are assumed to be its degradation products. (C) Densitometric quantification before vs. after IVM. Ab154888 antibody was used in both ICC and WB. a,b: means of labeling intensity by ICC with different letters differ (P < 0.01).
