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. 2020 Dec 23;10:573789. doi: 10.3389/fonc.2020.573789

Figure 5.

Figure 5

(A) qRT-PCR analysis of LINC02747 nuclear and cytoplasmic levels in 786-O and Caki-1 cells. U6 was used as a nuclear marker, and GAPDH was used as a cytosolic marker. (B) qRT-PCR detected the expression changes of the candidate miRNAs after overexpressing LINC02747 in 786-O and Caki-1 cells. (C) MiR-608 and LINC02747 binding sequences and LINC02747 mutated sequences. (D) A luciferase reporter assay was used to verify the direct binding between LINC02747 and miR-608. (E) The co-precipitated miR-608 was subjected to qRT-PCR for LINC02747 RIP experiments. (*** P < 0.001, ** P < 0.01, * P < 0.05).