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. 2020 Dec 23;11:603831. doi: 10.3389/fimmu.2020.603831

Figure 1.

Figure 1

Maternal DEHP effect on allergic lung inflammation in OVA-immunized F1 and F3 offspring. F1 (A, B) or F3 neonates (C, D) from vehicle or DEHP-exposed F0 female mice were immunized with or without OVA as indicated. Then all the mice were re-challenged with OVA aerosol, as described in Supplementary Figure 1C. Cell subsets (A, C) and cytokines (B, D) in BALFs by flow cytometry and ELISA, respectively. Results are shown as mean ± SEM. F1: Vehicle/PBS, n = 7 (three dams); Vehicle/OVA, n = 8 (three dams); DEHP/PBS, n = 6 in (A) or 4 in (B) (two dams); DEHP/OVA, n = 7 in (A) or 6 in (B) (three dams). F3 in (C): Vehicle/OVA, n = 15 (five dams); DEHP/OVA, n = 18 (five dams). F3 in (D): Vehicle/OVA, n = 23 (six dams); DEHP/OVA, n = 16 (six dams). *p-value < 0.05; **p-value < 0.01; ***p-value < 0.001 by one-way ANOVA followed by Tukey’s multiple comparison test (A, B) or by Mann-Whitney U test (C, D). The number of offspring (n) are pooled from at least two independent breeding.