TABLE 2.
Kinases modulated by the mutant receptors in comparison to the WT after 10 min of stimulus with AngII.
| Modulated proteins | Fold change (spots quantified in mutant receptor membrane/spots quantified in WT membrane) | ||
|---|---|---|---|
| I103T | A244S | I103T-A244S | |
| p38α | 1.39 ▲ | 1.10 | 1.23 |
| ERK1/2 | 1.85 ▲ | 1.55 ▲ | 1.03 |
| GSK-3α/β | 1.05 | 0.90 | 0.67 ▼ |
| AMPKα1 | 1.32 ▲ | 0.73 | 0.73 |
| AKT1/2/3 (S473) | 1.17 | 0.96 | 0.62 ▼ |
| CREB | 0.69 ▼ | 0.97 | 0.70 ▼ |
| HSP27 | 0.70 ▼ | 0.87 | 0.46 ▼ |
| β-Catenin | 0.98 | 1.17 | 0.58 ▼ |
| c-jun | 1.31 ▲ | 1.37 ▲ | 1.20 |
| Src | 0.67 ▼ | 0.90 | 0.57 ▼ |
| p70 S6 kinase | 1.03 | 1.32 ▲ | 1.06 |
| eNOs | 1.16 | 1.24 | 1.42 ▲ |
| Fyn | 0.48 ▼ | 0.85 | 0.60 ▼ |
| Yes | 0.60 ▼ | 0.84 | 0.74 |
| Fgr | 0.56 ▼ | 0.84 | 0.70 ▼ |
| STAT6 | 1.11 | 1.22 | 1.37 ▲ |
| p27 | 1.06 | 1.30 ▲ | 1.03 |
| WNK1 | 1.10 | 1.33 ▲ | 0.86 |
HEK293T protein cell lysates expressing wild-type AT1R or mutant receptors were stimulated with Ang II and incubated with the phosphokinase array membranes. Results were plotted using the ratio between spots quantified in mutant receptors membrane/spots quantified in WT receptor membrane. Only kinases or substrates with intensity changes of ±30% were considered to be affected by the condition (indicated by a black triangle. ▲: up-modulation ▼: down-modulation).