Figure 5.

3D Printing with composite hydrogel and fabrication of cancer spheroid containing Tumor-on-a-Chip. (A) Schematic illustration of 3D printing of a cancer spheroid containing flow chamber for cancer invasion and metastasis study. (B) Chip in 3D printing with BioX bio-printer (CellInk). (C) Upper panels are the schematic illustrate of the processing of the flow chip. Chip was first printed with F127 as a sacrificial layer. Then the L-G-C was printed and photo-crosslinked in the center of chip with cancer spheroids embedded, and the outer layer of the chip. The F127 layer was dissolved with phosphate buffer forming hollow channels for perfusion and the glass sealing was performed. Lower panels are the actual photo for the printed chip at each steps.