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. 2021 Jan 6;16:6. doi: 10.1186/s13018-020-02041-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Knockdown of lncRNA LEF-AS1 could decrease the LPS-induced microglia cells apoptosis. a The expression level of lncRNA LEF-AS1 in si-NC and si-LEF-AS1 groups. b Cell viability in LPS and/or transfected with si-LEF-AS1. c Apoptosis ratio in LPS and/or transfected with si-LEF-AS1 measured by Annexin V/FITC apoptosis assay. d Western blot assay measuring protein expression of apoptotic markers (Bax, Bcl-2, and cleaved-caspase-3) in microglia cells transfected with si-LEF-AS1. e Inflammatory cytokines (TNF-α and IL-6) expression levels in microglia cells transfected with si-LEF-AS1. *P < 0.05