Table 1.

The primers for each gene

Gene	Sequence
GAPDH	forward: 5′-GAAGATGGTGATGGGATTTC-3′
	reverse: 5′-GAAGGTGAAGGTCGGAGT-3′
U6	forward: 5′-CTCGCTTCGGCAGCACA-3′
	reverse: 5′-AACGCTTCACGAATTTGCGT-3′
LEF-AS1	forward: 3′-TGT GAC TCC AGA GGC GGA AC-5′
	reverse: 3′-AGG AGG CGG CAA GAA GAA GG-5′
miR-222-5p	forward: 5′-TGGCCTCGAGATGTGCTTCAG-3′
	reverse: 5′-TCTCCTTGGCGGCCGCACTTCCTTC-3
RAMP3	forward: 5′-ACACCCGTGAGAGAGACTTG-3′
	reverse: 5′-AAGTCAGTCGGGAAGGAAGG-3′