Figure 2.

HDAC4 and HDAC5 down-regulated ncx3 gene and protein expression. (a, b) ncx3 gene expression and representative WB with quantification in cortical neurons transfected with the following constructs: (1) Empty Vector, (2) HDAC4 Vector, (3) HDAC5 Vector, (4) HDAC7 Vector, and (5) HDAC9 Vector. (c, d) ncx3 gene expression and representative WB with quantification in cortical neurons transfected with the following siRNAs: (1) siCTL, (2) siHDAC4, (3) siHDAC5, (4) siHDAC7, and (5) siHDAC9. The cells were processed after 24 h of transfection for qRT-PCR and immunoblotting (n = 5). *p ≤ 0.05 versus empty vector or siCTL, one-way ANOVA, followed by Tukey’s multiple comparison test.