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. 2019 Nov 7;40(10):2081–2097. doi: 10.1177/0271678X19884742

Figure 4.

Figure 4.

DREAM, HDAC4, and HDAC5 bound to ncx3 brain promoter, determining its sequence deacetylation in the periischemic temporoparietal cortex of tMCAO rats. (a–c) Representative WB with quantification of DREAM, HDAC4, and HDAC5 in cortex of rats subjected to 24 h and 48 h of tMCAO (n = 5). *p < 0.05 versus Sham, one-way ANOVA, followed by Tukey’s multiple comparison test. Confocal double labeling experiments displaying the co-expression of DREAM (d), HDAC4 (e), and HDAC5 (f) with the neuronal marker NeuN in the ipsilateral temporoparietal cortex of sham-operated animals (left panels) and in the ipsilateral periischemic temporoparietal cortex 24 h after tMCAO surgery (right panels). Dashed lines delimit the cortical ischemic core and periischemic region. Scale bars 100 µm. ChIP analysis of the ncx3 brain promoter performed with: (g) anti-DREAM, (h) anti-HDAC4, (i) anti-HDAC5, and (j) anti-Acetylated-Histone H4 in cortex of rats subjected to 24 h of tMCAO. Binding activity of DREAM, HDAC4, HDAC5 and acetylation status of Histone H4 are graphically represented as the percentage of sham-operated animals (n = 5). *p ≤ 0.05 versus Sham, Student t test .