Fig. 6.

MiR-542-3p alleviated OGD induced glial cell damage. HA1800 cells were incubated with exo-miR-NC/exo-miR-542-3p and treated with or without OGD for 2 h and transfected with TLR4 plasmid at the same time. a MiR-542-3p expression in HA1800 cells was detected using qRT-PCR. b MTT assay for cell viability. c TUNEL assay for cell apoptotic level. Scale bar 20 μm. d ROS assay for the production of ROS in HA1800 cells. Scale bar 25 μm. e Inflammatory factors TNFα and MCP-1 were detected using qRT-PCR. f Western blot for the protein expression of TLR4, TNFα, and IL 6. Data are mean ± SD; *P < 0.05, **P < 0.01. All experiments were repeated in triplicate