Table 4.

Calculated values obtained from C_T values of log₁₀ dilutions of spiked sewage and MQ water samples.

Gene	Efficiency (%)		Slope		R2 value
	Sewage	MQ	Sewage	MQ	Sewage	MQ
E gene	94.54	89.47	−3.4601	−3.6030	0.9750	0.9685
N gene	95.87	92.71	−3.4251	−3.5099	0.9657	0.9995
ORF1ab	94.13	87.11	−3.4710	−3.6752	0.9465	0.9711
Average	94.85	89.76	−3.45	−3.60	0.96	0.98