(b) Refinement.

		Insulin ED data (data sets 3–7 merged)		Thermolysin		Thaumatin
	Insulin X-ray data	Uncorrected data	Corrected for dynamical scattering	Uncorrected data	Corrected for dynamical scattering	Uncorrected data	Corrected for dynamical scattering
Resolution range (Å)	24.20–2.30 (2.36–2.30)	30.30–3.25 (3.33–3.25)	30.30–3.25 (3.33–3.25)	37.52–3.26 (3.34–3.26)	37.49–3.26 (3.34–3.26)	12.34–2.76 (2.83–2.76)	12.34–2.76 (2.83–2.76)
No. of reflections
Work set	3213 (217)	1019 (64)	1030 (63)	4293 (262)	4293 (262)	4364 (108)	4364 (108)
Free set	180 (9)	108 (4)	97 (5)	237 (12)	237 (12)	231 (3)	231 (3)
No. of atoms	805	770	770	2438	2438	1551	1551
No. of reflections/No. of parameters	0.998	0.334	0.334	0.440	0.440	0.703	0.703
R factor	0.166	0.194	0.187	0.214	0.187	0.283	0.255
R work	0.162 (0.130)	0.181 (0.349)	0.178 (0.296)	0.210 (0.347)	0.184 (0.213)	0.281 (0.160)	0.253 (0.323)
R free	0.238 (0.333)	0.319 (0.501)	0.272 (0.338)	0.292 (0.541)	0.238 (0.416)	0.320 (0.176)	0.295 (0.276)
σ, bonds (Å)	0.008	0.006	0.006	0.007	0.006	0.005	0.005
σ, angles (°)	1.174	1.034	1.011	1.156	1.057	1.005	1.008

^†Only one crystal diffracted to 2.76 Å resolution (see Supplementary Table S3 for details). The completeness of the thaumatin data is 85.6% at 3.19 Å resolution, 81.9% at 3.09 Å resolution and 22.6% in the 3.09–2.76 Å resolution shell.