Fig. 1.

Frequencies and HLA-DR expression of primary blood DC subsets. Cells were gated on viable mononuclear cells (PBMCs) and then further gated on singlets. Blood DCs were identified as Lin⁻ HLA-DR⁺ cells (DCs) and further divided into CD16⁺ and CD16⁻, which were gated on CD1c⁺ and CD141⁺ DCs. CD1c/CD141 double-negative DCs were gated on CD303 to identify pDCs (a). Blood DC counts (b) and HLA-DR expression as MFI (c) were quantified comparing uninfected (n = 27) and asymptomatic P. falciparum-infected (n = 8) Malian adults. Each circle represents one individual donor at one time point; bars represent mean with standard deviation. P-value by unpaired t-test. MFI median fluorescence intensity