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. 2020 Dec 23;10:606574. doi: 10.3389/fonc.2020.606574

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Copyright © 2020 Acerbi, Pollo, De Laurentis, Restelli, Falco, Vetrano, Broggi, Schiariti, Tramacere, Ferroli and DiMeco

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Diagnostic partially concordant case: right parieto-occipital GBM (case n. 14). (A) Neuronavigation MR images showing the site of “A” biopsy sampling in the GBM core. (B) Intraoperative view during tumor removal with SF-guided technique. Yellowish fluorescent areas under Y560 filter activation of the surgical microscope correspond to tumor tissue (site of “A” sampling, black arrow). (C, D) CONVIVO® and permanent section images, respectively, within the biopsy sample, showing an area of tumor tissue with prevalent necrotic aspects, such as low cellular density, prevalence of amorphous tissue on an intermediate fluorescence background (C), confirmed as a low-cellular density necrotic area within the permanent section sample (red-dotted lines in D).