KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| monoclonal antibody against Pericardin | Developmental Studies Hybridoma Bank Iowa City, IA 52242. | DSHB Cat # EC11 RRID:AB_528431 |
| 0S proteasome subunits a1,2,3,5,6 & 7 | Enzo Life Sciences, Farmingdale, NY | Cat # BML-PW8195 RRID:AB_10541045 |
| Mouse anti-GFP | Invitrogen, Carlsbad, CA, USA | Cat # 33-2600 RRID:AB_2533111 |
| anti-rabbit IgG conjugated with Alexa Fluor 488 | Invitrogen, Carlsbad, CA, USA | Cat # Cat # R37116 RRID:AB_2556544 |
| anti-mouse IgG conjugated with Alexa Fluor 594 | Invitrogen, Carlsbad, CA, USA | R37121 RRID:AB_2556549 |
| Phalloidin-Alexa Fluor 594 | Molecular Probes, Eugene, OR | Cat # A12381 RRID:AB_2315633 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Flynap | Carolina Biological Supply Co, Burlington, NC | #173010 |
| Critical Commercial Assays | ||
| miRNeasy Mini kit | QIAGEN | Cat. No. 217004 |
| Illumina TruSeq™ RNA sample preparation kit | Illumina, San Diego, CA) | RS-122-2001 |
| Deposited Data | ||
| Drosophila melanogaster genome Ensembl.BDGP5 | ensembl | http://uswest.ensembl.org/Drosophila_melanogaster/Info/Index |
| Kyoto Encyclopedia of Genes and Genomes | https://www.kegg.jp/ | RRID:SCR_012773 |
| NASA Gene Labs | This paper | https://genelab-data.ndc.nasa.gov/genelab/accession/GLDS-347 |
| Experimental Models: Organisms/Strains | ||
| Canton S Wildtype Strain | Bloomington Drosophila Stock Center | BDSC Cat# 64349, RRID:BDSC_64349 |
| Sei/hERG mutant | Dr. Barry Ganetsky | N/A |
| PolyQ46-GFP line | Dr. Girish Melkani | N/A |
| Software and Algorithms | ||
| SOHA Semi Automated Optical Heartbeat Analysis | http://www.sohasoftware.com | N/A |
| Axiovision software | Zeiss | RRID:SCR_002677 |
| Weka Trainable Segmentation plugin in ImageJ | https://www.cs.waikato.ac.nz/ml/weka/ | RRID:SCR_001214 RRID:SCR_001935 |
| FASTX-Toolkit | Hannon Lab, Cold Spring Harbor, NY, USA) | RRID:SCR_005534 |
| BEDTools | https://github.com/arq5x/bedtools2 | RRID:SCR_006646 |
| AltAnalyze | http://www.altanalyze.org/ | RRID:SCR_002951 |
| Top Hat | http://ccb.jhu.edu/software/tophat/index.shtml | RRID:SCR_013035 |
| Bow Tie | http://bowtie-bio.sourceforge.net/index.shtml | RRID:SCR_005476 |
| Other | ||
| 9300 EM-CCD cameras | Hamamatsu Corp. | https://www.hamamatsu.com/eu/en/product/cameras/emccd-cameras/index.html |
| Zeiss Apotome microscope | Zeiss | https://www.zeiss.com/microscopy/us/products/imaging-systems/apotome-for-biology.html |
| Agilent 2200 Tapestation system | Agilent, Santa Clara, CA | https://www.agilent.com/en/product/automated-electrophoresis/tapestation-systems |
| Illumina HiSeq 2000 | Illumina, San Diego, CA) | https://www.illumina.com |