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. 2020 Dec 8;20(8):foaa066. doi: 10.1093/femsyr/foaa066

Figure 3.

Figure 3.

The overexpression of CgVHT1 increases intracellular fitness of Cgvhr1Δ. (A), Candida glabrata wt (black letters), mutant (Cgvhr1∆, red letters) and corresponding overexpression strains (+LYS21prom-VHT1, +TEF1prom-VHT1, +PDC1prom-VHT1) were cultivated in YPD for 90 min at 37°C and 180 rpm. Target gene expression was analyzed by qRT-PCR and normalized to ACT1 and EFB1. Expression is shown relative to the Cg wt strain. Data are shown as mean ± SD of two independent experiments. (B), Candida glabrata wt (black letters), mutant (vhr1∆, red letters), and corresponding overexpression strains (+pLYS21-VHT1, +pTEF1-VHT1) were pre-cultured in YPD + 2 mg/L biotin before confrontation with MDMs. Survival of C. glabrata strains after 3 h co-incubation with MDMs at an MOI of 1 is shown as survival in % of inoculum (mean ± SD). Each single dot represents one individual blood donor (in total at least two independent experiments with four donors each). For statistical analysis, a repeated measures ANOVA with Bonferroni's multiple comparison test was performed comparing all strains (*P ≤ 0.05, **P ≤ 0.01).