Table 1.
Strains used in this study.
| Strains | Internal ID | Designation/genotype | Reference |
|---|---|---|---|
| C. glabrata | |||
| ATCC2001 | C94 | Clinical isolate | (Schwarzmüller et al. 2014) |
| Cgtrp1∆ | G17 | Parental strain (derivative of ATCC2001) trp1::FRT | (Jacobsen et al. 2010) |
| Cgtrp1∆ + CgTRP1 (Cg wt) | G203 | Isogenic wild type trp1::TRP1 | (Sprenger et al. 2020) |
| Cg wt + OVA | G205 | trp1::(TRP1-CgTEF1prom(-1 to -1000)-OVA) | This study |
| Cg wt + CgLYS21p-CgVHT1 | G247 | trp1::(TRP1-CgLYS21prom(-1 to -1157)- CAGL0K04609g-CAGL0K04609gterm) | This study |
| Cg wt + CgTEF1p-CgVHT1 | G249 | trp1::(TRP1-CgTEF1prom(-1 to -1000)-CAGL0K04609g-CAGL0K04609gterm) | This study |
| Cg wt + CgPDC1p-yEGFP | G257 | trp1::(TRP1-CgPDC1prom(-1 to -973)-yEGFP-CgHIS3term) | This study |
| Cg wt + CgLYS21p- yEGFP | G259 | trp1::(TRP1-CgLYS21prom(-1 to -1157)-yEGFP-CgHIS3term) | This study |
| Cg wt + CgTEF1p- yEGFP | G261 | trp1::(TRP1-CgTEF1prom(-1 to -1000)-yEGFP-CgHIS3term) | This study |
| Cg wt + CgTEF1p-mCherry | G325 | trp1::(TRP1-CgTEF1prom(-1 to -1000)-mCherry- CgCTA1term) | This study |
| Cgtrp1∆vhr1∆ | G237 | trp1::FRT CAGL0M12496g::NAT1 | This study |
| Cgvhr1∆ | G241 | trp1::TRP1 CAGL0M12496g::NAT1 | (Sprenger et al. 2020) |
| Cgvhr1∆ + CgLYS21p-CgVHT1 | G253 | CAGL0M12496g::NAT1 trp1::(TRP1-CgLYS21prom(-1 to -1157)- CAGL0K04609g-CAGL0K04609gterm) | This study |
| Cgvhr1∆ + CgTEF1p-CgVHT1 | G255 | CAGL0M12496g::NAT1 trp1::(TRP1-CgTEF1prom(-1 to -973)- CAGL0K04609g-CAGL0K04609gterm) | This study |
| Cgtrp1∆dur1,2∆ | G330 | trp1::FRT CAGL0M05533g::NAT1 | This study |
| Cgdur1,2∆ | G366 | trp1::TRP1 CAGL0M05533g::NAT1 | This study |
| Cgdur1,2∆ + CgDUR1,2 | G367 | CAGL0M05533g::NAT1; trp1::(TRP1-CAGL0M05533g) | This study |
| Cgdur1,2∆ + CgDUR1,2K1798R | G368 | CAGL0M05533g::NAT1; trp1::(TRP1-CAGL0M05533g with codon 1798: AAA → AGA) | This study |
| C. albicans | |||
| BWP17 | M130 | Parental strain (derivative of SC5314) ura3::λimm434/ura3::λimm434 his1::hisG/his1::hisG arg4::hisG/arg4::hisG | (Wilson, Davis and Mitchell 1999) |
| BWP17 CIp30 (Ca wt) | M1477 | Isogenic wildtype ura3::λimm434/ura3::λimm434 his1::hisG/his1::hisG arg4::hisG/arg4::hisG RPS1/rps1::CIp30-URA3-HIS1-ARG4 | (Citiulo et al. 2012) |
| Caura3∆/∆dur1,2∆/∆ | M2671 | orf19.7468::ARG4/orf19.7468::HIS1 | This study |
| Cadur1,2∆/∆ | M2672 | orf19.7468::ARG4/orf19.7468::HIS1 RPS1/rps1::CIp10-URA3 | This study |
| Cadur1,2∆/∆ + CgDUR1,2 | M2674 | orf19.7468::ARG4/orf19.7468::HIS1 RPS1/rps1::CIp10-URA3-orf19.7468 | This study |
| Cadur1,2∆/∆ + CgDUR1,2K1779R | M2721 | orf19.7468::ARG4/orf19.7468::HIS1 RPS1/rps1::CIp10-URA3-orf19.7468 with (codon 1779: AAA → AGA) | This study |
| E. coli | |||
| Stellar™ Competent Cells | F–, endA1, supE44, thi-1, recA1, relA1, gyrA96, phoA, Φ80d lacZΔ M15, Δ (lacZYA-argF) U169, Δ (mrr—hsdRMS—mcrBC), mcrA, λ– | In-Fusion® HD Cloning Plus Kit |