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. 2020 Dec 8;20(8):foaa066. doi: 10.1093/femsyr/foaa066

Table 2.

Plasmids used in this study.

Plasmid Used for Reference
pFA-HIS1 Amplification of CaHIS1 cassette (Gola et al. 2003)
pFA-ARG4 Amplification of CaARG4 cassette (Gola et al. 2003)
CIp10 Integration of CaURA3 into RPS1 locus (Murad et al. 2000)
pTRP1 Amplification of CgTRP1 cassette, Basis for TRP1 complementation constructs (Sprenger et al. 2020)
pCN-PDC1-GFP CEN/ARS episomal plasmid for yEGFP expression in C. glabrata under control of the PDC1 promotor (Zordan et al. 2013)
pYC56 Recipient integrative vector harboring mCherry (Yánez-Carrillo et al. 2015)
pTRP1-LYS21prom-yEGFP Amplification of CgTRP1 and yEGFP under control of the LYS21 promotor This study
pTRP1-LYS21prom-VHT1 Amplification of CgTRP1 and CAGL0K04609g under control of the LYS21 promotor This study
pTRP1-PDC1prom-yEGFP Amplification of CgTRP1 and yEGFP under control of the PDC1 promotor This study
pTRP1-PDC1prom-VHT1 Amplification of CgTRP1 and CAGL0K04609g under control of the PDC1 promotor This study
pTRP1-TEF1prom-yEGFP Amplification of CgTRP1 and yEGFP under control of the TEF1 promotor This study
pTRP1-TEF1prom-VHT1 Amplification of CgTRP1 and CAGL0K04609g under control of the TEF1 promotor This study
pTRP1-TEF1prom-OVA Amplification of CgTRP1 and OVA under control of the TEF1 promotor This study
pTRP1-TEF1prom-mCherry Amplification of CgTRP1 and mCherry under control of the TEF1 promotor This study
CIp10-CaDUR1,2 Integration of CaURA3 and CaDUR1,2 (orf19.780) into RPS1 locus This study
CIp10-CaDUR1,2 (K1779R) Integration of CaURA3 and orf19.780 (lysine 1779 mutated to arginine) into RPS1 locus This study
pTRP1-CgDUR1,2 Amplification of CgTRP1 and CgDUR1,2 (CAGL0M05533g) This study
pTRP1-CgDUR1,2 (K1798R) Amplification of CgTRP1 and CAGL0M05533g (lysine 1798 mutated to arginine) This study