Figure 5.

CD70 expression by hematopoietic rather than non-hematopoietic cell types contribute to thymic Treg development. NOD and Cd70^−/− mice were lethally irradiated and reconstituted with BM cells from CD70-deficient or -intact mice. Thymus and spleen were analyzed eight weeks after BM reconstitution for the (A) frequency and (B) number of total Tregs as well as the frequency of Helios⁺ Tregs (C) and Helios⁻ Tregs (D). The results represent two independent experiments with 3 to 5 mice per group. Each symbol represents an independent biological replicate comprised of a single recipient mouse reconstituted with BM from a single donor animal. Data were analyzed by the Mann-Whitney test. *P < 0.05, **P < 0.01. Actual P valves are provided when 0.1>p-value>0.05.