Table 1.
Flow cytometric analysis of leukocyte populations in spleens of 8- to 10-week-old female (F) and male (M) NOD mice carrying none, one or both copies of Cd70 mutant gene.
| Cd70+/+ F | Cd70+/− F | Cd70−/− F | Cd70+/+ M | Cd70+/− M | Cd70−/− M | |
|---|---|---|---|---|---|---|
| Lymphocytes (x107) | 7.24±0.45 | 6.57±0.57 | 5.64±0.51 | 5.98±0.35 | 5.59±0.24 | 5.85±0.60 |
| Conventional DC (%) | 1.91±0.13 | 1.93±0.12 | 1.94±0.13 | 1.23±0.05 | 1.68±0.11 | 1.57±0.14 |
| Conventional DC (x106) | 0.83±0.08 | 0.93±0.12 | 1.16±0.06 | 0.60±0.04 | 0.77±0.05 | 0.84±0.05 |
| Plasmacytoid DC (%) | 1.47±0.09 | 1.41±0.06 | 1.38±0.15 | 1.06±0.05 | 1.11±0.10 | 0.96±0.02 |
| Plasmacytoid DC (x106) | 0.62±0.05 | 0.66±0.09 | 0.92±0.12 | 0.50±0.03 | 0.49±0.05 | 0.51±0.04 |
| Eosinophils (%) | 7.82±1.11 | 7.90±0.60 | 8.37±1.24 | 5.86±0.28 | 4.99±0.44 | 5.93±0.95 |
| Eosinophils (x106) | 0.12±0.02 | 0.13±0.02 | 0.16±0.03 | 0.08±0.01 | 0.08±0.01 | 0.13±0.02 |
| Macrophages/monocytes (%) | 38.66±1.84 | 36.64±1.82 | 40.85±1.63 | 50.28±2.34 | 49.70±1.14 | 47.82±1.29 |
| Macrophages/monocytes (x106) | 0.61±0.07 | 0.64±0.04 | 0.87±0.15 | 0.68±0.06 | 0.79±0.06 | 1.01±0.05 |
| Neutrophils (%) | 48.77±1.91 | 50.85±2.24 | 45.70±1.36 | 40.42±2.53 | 42.10±0.96 | 42.43±1.37 |
| Neutrophils (x106) | 0.75±0.06 | 0.89±0.09 | 0.95±0.10 | 0.55±0.07 | 0.67±0.05 | 0.89±0.03 |
Single-celled, live lymphocytes were identified according to size and granularity. Dendritic cells (DCs) were identified as CD11c+ cells after gating single live leukocytes. Different DCs subsets were distinguished from each other according to pCDA1 surface expression. Eosinophils, neutrophils, macrophages and monocytes were identified as CD11b+ single live leukocytes with respectively high and low granularity according to side scatter and surface expression of Ly6G. The number of immune cells were determined by calculating the absolute number of each cell type from fluorescent-activated cell analysis profiles and total cell numbers in the spleen. Values represent mean ± SEM. At least 10 individual mice were examined per group. No significant differences (p > 0.05) were detected between the indicated genotypes for any of the immune cell populations according to the Mann-Whitney test.