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. 2021 Jan 6;16(1):e0243862. doi: 10.1371/journal.pone.0243862

Fig 1. ST266 increases Schwan cell proliferation following 24-hours starvation conditions.

Fig 1

Media of Schwan cells cultured in normal growth media (NGM) was replaced by minimal starvation medium for 24-hours and subsequently replaced by either NGM, IMDM, STM100 (base medium), ST266 or filtrate of ST266 below 50- or 30- kDa cutoff. (a) After 24-hours treatment, cell proliferation was determined by extracellular reduction of WST-8, with results shown as a ratio of sample absorbance to the NGM value for each plate. (b) Representative light micrograph images of cultured murine Schwann cells following starvation and a 24-hours treatment demonstrate relative presence of healthy, elongated Schwann cells at 20X (scale bars = 200 μm) and 40X (scale bars = 100 μm) magnification. Images illustrating the development of color from CCK-8 assay in Schwann cell cultures. Corresponding cell viability is listed below each representative set of images. Data shown as mean + SEM from three separate experiments with total of n = 12 per group (**p < 0.0001 and *p < 0.05).