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Fluorescence detection of MTB/NTM-LAMP results for positivity of MTB (purple, Ct value for MTB: 21.0, A), B. NTM (red, Ct value for NTM: 34.58, B), and Negative (C), respectively. Abbreviations: LAMP; loop mediated isothermal amplification, MTB; Mycobacterium tuberculosis, NTM; non-tuberculosis mycobacterium, GAPDH; human genomic DNA for Glyceraldehyde 3-phosphate dehydrogenase, IC; internal control, RFU; Relative fluorescence units, Ct; Cycle thresholds.
