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. 2021 Jan 6;16(1):e0244753. doi: 10.1371/journal.pone.0244753

Table 3. Real-time PCR result distribution according to the specimen (N = 333).

Specimen Real-time MTB PCR positive* Real-time PCR NTM positive Negative** Total
AFB stain Neg 1+ 2+ 3+ 4+ total Neg 1+ 2+ 3+ 4+ total Neg
Pulmonary Bronchial washing 17(16) 9(8) 2 2 30(28) 10 5(4) 7(6) 3 25(23) 52 107(103)
Sputum 19(18) 5 2(1) 1 2 29(27) 79(73) 3 6(5) 3 4(3) 95(87) 33(32) 157(146)
Total 36(34) 14(13) 4(3) 1 4 59(55) 89(83) 8(7) 13(11) 3 7(6) 120(110) 85(84) 264(249)
Extrapulmonary Cerebrospinal fluid 0 0 14(13) 14(13)
Joint or bone aspiration 1 1 1 1 10 12
Pleural fluid or ascites 1 1 1 1 23(22) 25(24)
Lymph node or tissue biopsy 6(5) 6(5) 0 1 7(6)
Pus 3(2) 2(1) 5(3) 0 4 9(7)
Urine 0 0 1 1
Stool 0 1 1 0 1
Total 11(9) 2(1) 0 0 0 13(10) 2 1 0 0 0 3 53(51) 69(64)

*Total 19 of TB PCR positive samples showed no growth in TB culture results.

** All of these samples showed negative for AFB stain and no growth for TB culture results.

In case of duplicate collection, the number of patients were indicated in parentheses.

Abbreviations: Neg, negative; MTB, Mycobacterium tuberculosis; AFB, acid fast bacilli; NTM, Non-tuberculosis Mycobacterium.