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. 2020 Aug 27;245(17):1552–1559. doi: 10.1177/1535370220950639

Figure 2.

Figure 2.

Arecoline fed from mid-life stages extends lifespan in C. elegans. Survival curves of control (blue) and 0.2 mM arecoline-treated (red) worms from (a) whole adulthood, P < 0.0001, and (b) early adulthood from Day 1 to Day 3, P = 0.0002. Survival curves of animals treated with control vehicle (blue) and arecoline (green) from mid-life stages: (c) Day 3 to Day 5, P = 0.0002, (d) Day 4 to Day 6, and (e) Day 5 to Day 7. (f) Survival curves of control (blue) and arecoline-treated (red) worms from adult Day 6 to Day 8. The arecoline groups of animals were maintained on NGM plates with 0.2 mM arecoline for the above-mentioned periods. P-values represent results of log-rank calculation. The graphs are representative of two or three independent experiments. Detail statistical data are included in Table S1.