Figure 5.
Peptidoglycan from L. rhamnosus MLGA increases antibacterial activity of chicken PBMCs (a) and splenocytes (b). Chicken PBMCs or splenocytes were treated with or without different concentrations of L. rhamnosus MLGA-derived peptidoglycan for 6 h. Cell lysates were them prepared and incubated with S. Enteritidis (ATCC 13076) for 9 h at 37°C. Bacterial turbidity at OD590 nm was measured as an indication of the bacterial density. S. Enteritidis was also directly incubated with different concentrations of L. rhamnosus MLGA-derived peptidoglycan in cell culture medium alone without chicken primary cells (c) or incubated with supernatants from co-culture of PBMCs or splenocytes with L. rhamnosus MLGA-derived peptidoglycan (d). Values are given as the mean ± SD of two to three independent experiments. **P < 0.01 compared with control group.