Table 1.

The primer and probe sequences of AvBD9 and cytokines for real-time PCR reactions.

Gene name	Sequence (5′- 3′)	GenBank accession no.	Product size (bp)
AvBD9	Forward: CACCTTAGCATGCAGGCAGA Reverse: GCCCATTTGCAGCATTTCA Probe: FAM-TTGCATGCCGTGCTCCTTCAGTTG-BHQ1	NM_001001611	111
IL-1β	Forward: TGACCCGCTTCATCTTCTACC Reverse: GGTCGGGTTGGTTGGTGA Probe: FAM-CACGCGCTTCGAGTCGGCC-BHQ1	AJ245728	138
IL-8	Forward: GCTGGTAAAGATGGGGAATGA Reverse: CGTCAGCTTCACATCTTGAATG Probe: FAM-CATTAGCACTCATTCTAAGTTCATCCACCC-BHQ1	AJ009800	94
IL-12p40	Forward: GTAACCTGCAGCAGCCCTG Reverse: GTCGGCTGGTGCTCTTCG Probe: FAM-CTGTGACTGAATACACTGCCCAGTGCC-BHQ1	NM_213571.1	107
GAPDH	Forward: GGTGCTAAGCGTGTTATCATCTC Reverse: CATGGTTGACACCCATCACAA Probe: FAM-CTCCCTCAGCTGATGCCCCCATG-BHQ1	NM_204305	70

AvBD9: avian β-defensin 9.