Figure 5.

Percentage of Tregs (CD4⁺CD25⁺Foxp3⁺ cells) in CD4⁺ T cells from NCs in vitro. PBMC were cultured with 10% FBS (containing 1 µg/ml anti-CD3 and 1 µg/ml anti-CD28). (a) Representative flow cytometer scatter diagrams of the proportion of Tregs in CD4⁺ T cells were shown in PBMC from NC cultured alone, and PBMC from NC co-cultured with MDSCs from MDS. The rectangle R3 presents the percentage of CD4⁺CD25⁺ T cells in CD4⁺ T cells, and the upper-right quadrant represent the percentage of CD25⁺Foxp3⁺ cells in CD4⁺CD25⁺ T cells. (b) The histogram represents the percentage of CD4⁺CD25⁺Foxp3⁺ cells in CD4⁺ T cells from NCs, lower-risk MDS and higher-risk MDS. (c) The histogram represents the percentage of CD4⁺CD25⁺Foxp3⁺ cells in CD4⁺ T cells from PBMC from NCs cultured alone and PBMCs from NCs co-cultured with MDSCs from MDS. *P < 0.05. Treg: regulatory T cells.