Fig. 2.

hESC-MSCs exhibit preferable characteristics in multilineage differentiation, karyotyping and proliferation. a The phase contrast images of hESC-MSC-derived adipocytes with Oil red O staining. Scale bar = 100 μm. b qRT-PCR analysis of the adipogenic-associated genes. c Alizarin Red staining of hESC-MSC-derived osteoblasts. Scale bar = 100 μm. d qRT-PCR analysis of the osteogenic-associated genes. e Alcian Blue staining of hESC-MSC-derived chondrocytes. Scale bar = 100 μm. f qRT-PCR analysis of the chondrogenic-associated genes. g Karyotypic analysis of hPSC-MSCs with G-banded chromosome experiment. h Long-term in vitro Expansion potential of hPSC-MSCs for 12 passages by Pd assay. All data were shown as mean ± SEM (N = 3). *P < 0.05, **P < 0.01; NS, not significant