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. 2021 Jan 6;11:6. doi: 10.1186/s13578-020-00516-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — HA hydrogels enhanced in vitro chondrogenic differentiation of hESC-MSCs whereas minimally affected cell vitality. a Scanning electron microscope (SEM) analysis of HA hydrogels (HA) and HA/hESC-MSCs hydrogel (HA/hESC-MSCs). b Immunofluorescence analysis of F-ACTIN cytoskeleton of monolayer cultured hESC-MSCs and HA/hESC-MSCs. Scale bar = 20 μm. c FCM analysis of apoptotic population in hESC-MSCs and HA/hESC-MSCs. d The dynamic images of sections of HA/hESC-MSCs-derived chondrocytes with Alcian Blue staining. Scale bar = 100 μm. e qRT-PCR analysis of the chondrogenic-associated genes in the hESC-MSCs and HA/hESC-MSCs groups. f FCM assay of CD40⁺, CD80⁺ or CD86⁺ cells in hESC-MSCs or HA/hESC-MSCs. All data were shown as mean ± SEM (n = 6). *P < 0.05, **P < 0.01; NS, not significant