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. 2021 Jan 6;166(2):545–557. doi: 10.1007/s00705-020-04900-3

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© Springer-Verlag GmbH Austria, part of Springer Nature 2021

This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

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Fig. 3 — Levels of anti-influenza-virus antibodies in mouse sera one week after the last immunization. (A) Total IgG titers. (B) IgG1 titers. (C) IgG2a titers. (D) IgG2a/IgG1 ratio. Values for individual sera are expressed at 450 nm in each group (n = 3). Differences between mice immunized with γ-Flu+IL-28B plasmid and γ-Flu alone were statistically highly significant according to one-way ANOVA results (***, p < 0.001). The graphs also illustrate that the levels of influenza-virus-specific antibodies in all vaccinated and control groups differed considerably (p < 0.001).