Fig. 5.

Assessment of lymphoproliferative responses after in vitro restimulation of cultured spleen cells with γ-Flu (4 μg/ml). Three mice in each group were sacrificed 7 days after the third immunization, and splenocytes proliferation in response to antigen stimulation was estimated using an MTT assay. **indicates a statistically significant difference between mice that received γ-Flu + IL-28B plasmid and γ-Flu alone, as determined by one-way ANOVA (P < 0.01). Values are presented as the mean ± SD for three samples. Differences between all immunized and control groups were statistically highly significant (p < 0.001).