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. 2020 Dec 8;9:e62334. doi: 10.7554/eLife.62334

Figure 4. Correlative imaging in dendrites and spines.

(a) Confocal image of SiR-tubulin (green) and SiR700-actin (red). (b) STED image of SiR-tubulin (green) for the same zone as in (a). (c-e) SXRF element maps (P, S, Zn) from the region of interest framed in (a) and (b). (f) Overlay image of phosphorus (green) and sufur (red). (g) Overlay image of phosphorus (green) and zinc (red). (h) Overlay image of sulfur (green) and zinc (red). (i) Pearson’s correlation coefficients for the elements (P, S, Zn) in the roi. (j) Overlay image of STED SiR-tubulin (green) and zinc (red). Scale bars: 500 nm, except for (a) and (b) 1 µm. Color scales min-max values in ng.mm−2.

Figure 4—source data 1. Data for Pearson’s correlation coefficients.

Figure 4.

Figure 4—figure supplement 1. Multimodal imaging of dendrites and spines.

Figure 4—figure supplement 1.

(A) STED SiR-tubulin (green) and confocal SiR700-actin (magenta) (B) Synchrotron radiation X-ray PCI of the same area as (A). (C–E) SXRF element maps (P, S, Zn) from the same region as (A) and (B). (F–H) SXRF element maps (P, S, Zn) from the framed region one in (B). (I–L) SXRF element maps (P, S, Fe, Zn) from the framed region two in (B). Scale bars: 200 nm, except (A) and (B) 1 µm. Color scales min-max values in ng.mm−2.