Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Biological sample (Rattus norvegicus) | Primary rat hippocampal neurons | Janvier labs, St Berthevin, France | Freshly isolated from E18 Sprague-Dawley rats |
|
| Antibody | Mouse IgG1 anti-beta tubulin (mouse, monoclonal) | Sigma Aldrich | Sigma T4026 RRID:AB_477577 |
(1:3000) |
| Antibody | Goat anti Mouse IgG1 CF568 (goat, polyclonal) | Ozyme | Ozyme BTM20248 RRID:AB_10854985 | (1:500) |
| Chemical compound, drug | Phalloidin-Alexa647 | Thermo Fisher Invitrogen | A22287 | (1:40) |
| Chemical compound, drug | SiR-actin | Spirochrome | SC001 | 1 µM |
| Chemical compound, drug | SiR-tubulin | Spirochrome | SC002 | 1 µM |
| Chemical compound, drug | SiR700-actin | Spirochrome | SC013 | 1 µM |
| Chemical compound, drug | TPEN (N,N,N,N-Tetrakis(2-pyridylmethyl)ethylenediamine) | Sigma Aldrich | P4413 | 5 µM |
| Chemical compound, drug | Zn (zinc chloride) | Sigma Aldrich | Z0152 | 10 µM |
| Chemical compound, drug | cytosine- arabinofuranoside | Sigma Aldrich | C6645 | |
| Chemical compound, drug | Paraformaldehyde | EMS | EMS 15710 |
Used at 2 or 4% |
| Chemical compound, drug | BrainPhys medium | STEMCELL | 05790 | |
| Chemical compound, drug | Neurobasal medium | GIBCO | A3582901 | |
| Chemical compound, drug | Neurocult SM1 neuronal supplement | STEMCELL | 05711 | |
| Chemical compound, drug | poly-lysine | Sigma Aldrich | P2636 | |
| Chemical compound, drug | 2-methylbutane | Sigma Aldrich | M32631 | |
| Chemical compound, drug | ethanol | Sigma Aldrich | 39278 | |
| Commercial assay, kit | ReadyProbes cell viability imaging kit Blue/Green | Thermo-Fischer Scientific | R37609 | |
| Software, algorithm | R. software | R. Core Team | v 3.6.1 | https://www.R-project.org/ |
| Software, algorithm | R-studio software | R-studio | v1.2.5001 | http://www.rstudio.com/ |
| Software, algorithm | ImageJ software | ImageJ | http://imagej.nih.gov/ij/ | |
| Software, algorithm | PyMCA software | PyMCA (ESRF) | http://pymca.sourceforge.net/ | |
| Other | SN, silicon nitride membranes | Silson Ltd, Southam, UK | SiRN-5.0(o)−200–1.5-500 | Includes orientation frame |