(
A) Average number of voxels occupied by all ROIs belonging to each response type (as in
Figure 7F) across all 36 central brain neuropils for each condition (pC1-A or pC1-S activation, or controls), sorted from left to right by the amount of response type 2 following pC1-A activation. (
B) Distribution of mean ROIs' response (DF/F) per flies at t = 0 (stimulus offset), t = 3 min, and t = 6 min from response type 2 (see
Figure 7D) for pC1-A activation. Each ROI's activity was z-scored relative to the baseline; therefore, DF/F units are plotted in standard deviation (SD) relative to baseline activity. p-values: one-way ANOVA with Bonferroni corrections. (
C) Sensory-evoked versus optogenetically-evoked responses. Distribution of neural responses to pulse, sine, or white noise auditory stimuli at t
0 (auditory stimulus offset) form all auditory ROIs (n = 33 flies, 19,036 ROIs; see
Pacheco et al., 2021) - blue, compared with optogenetically-evoked responses at t
0 (light offset) for ROIs with either transient or persistent activity - black (data from
Figure 7C). Responses were z-scored relative to the baseline; therefore DF/F units are plotted in standard deviation (SD) relative to baseline activity (baseline = −8 to −0.5 s before auditory stimulus onset and −245 to −5 s for before optogenetic light onset).