Figure panel	Genotype	Additional information
1A	UAS-2xEGFP; Dsx-Gal4	Immunostaining
1C-D	w;R71G01-LexA/8xLexAop2-FLP; Dsx-Gal4/UAS > STOP > TNT	Express TNT in pC1-Int
1S1B	w;R41A01-LexA/8xLexAop2-FLP; Dsx-Gal4/UAS > STOP > TNT	Express TNT in pCd1 neurons
1C-D, 1S1B	w;+/8xLexAop2-FLP;Dsx-Gal4/UAS > STOP > TNT	Control for TNT expression in pC1-Int and in pCd1
1 F-G, 2, 4 F-H, 1S1D, 2S1, 2S2B-C, 2S3	w;R71G01-LexA/8xLexAop2-FLP; Dsx-Gal4/10xUAS > STOP > ReachR	Express ReachR in pC1-Int neurons; ATR+ experimental, ATR- control
1S1C, 2S2A	w;R41A01-LexA/8xLexAop2-FLP; Dsx-Gal4/10xUAS > STOP > ReachR	Express ReachR in pCd1 neurons; ATR+ experimental, ATR- control
4D-E	w;R71G01.AD/+;DSX.DBD/UAS-ReachR	Express ReachR in ‘pC1-s’ neurons (behavioral experiment); ATR+ for experimental, ATR- for control
4A-C, F, J	w;VT25602.AD/+;VT2064.DBD/10xUAS-ReachR	Express ReachR in ‘pC1-A’ neurons (behavioral experiment); ATR+ for experimental, ATR- for controls. pC1-A is similar to pC1dSS3 in Schretter et al., 2020.
4S1	w;UAS-2xEGFP/+;VT25602-Gal4/+	Express GFP in pC1d/e neurons for patch clamp recordings
3D (top), 3S2C	w;R71G01.AD/UAS-10x-IVS-myr::GFP;Dsx.DBD/+	Immunostaining (pC1-S)
3D (bottom), 3S2B	w;VT25602.AD/UAS-10x-IVS-myr::GFP;VT2064.DBD/+	Immunostaining (pC1-A)
3S2A	R71G01-LexA/8xLexAop2-FLP; Dsx-Gal4/UAS > STOP > myrGFP	Immunostaining (pC1-Int)
7B-E, 7S1A	w+NorpA[36],20xUAS- csChrimson.mVenus; R71G01.AD/R57C10-LexA; Dsx.DBD/8xLexAop-mCD8tdTomato, 13xLexAop-GCaMP6s	pC1-S activation, measure pan-neuronal Ca response; ATR+
7B-J, 7S1A-B	w+NorpA[36],20xUAS-csChrimson.mVenus; VT25602.AD/R57C10-LexA; VT2064.DBD/8xLexAop-mCD8tdTomato, 13xLexAop-GCaMP6s	pC1-A activation, measure pan-neuronal Ca response; ATR+
7B-E,I-J, 7S1A	w+NorpA[36],20xUAS-csChrimson.mVenus; R57C10-LexA/CyO;8xLexAop-mCD8tdTomato, 13xLexAop-GCaMP6s/TM6B,tb	Control; ATR+
8A-B, 8S1	10xUAS-Chrimson.tdTomato, 13LexAop2-GCaMP6S/W+NorpA[36], 20xUAS-csChrimson.mVenus; VT25602.AD/CyO;Dsx-LexA/TV2064.DBD	pC1-A activation, measure Ca response in Dsx+ neurons; ATR+
8A-B, 8S1	10xUAS-Chrimson.tdTomato,13LexAop2-GCaMP6s/+; Sp/CyO;Dsx-LexA/TM6B,tb	Control; ATR+
8C-E, 8S1	10xUAS-Chrimson.tdTomato,13LexAop-GCaMP6s/+; VT25602.AD/CyO; Fru-LexA/VT2064.DBD	pC1-A activation, measure Ca response in fru+ neurons; ATR+
8C-E	10xUAS-Chrimson.tdTomato,13LexAop-GCaMP6s; Sp/CyO;Fru-LexA/Tm6Tb	Control; ATR+