Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) |
NM91 | Coen et al., 2014 | A gift from Peter Andolfatto |
|
| Genetic reagent (D. melanogaster) |
Dsx-Gal4 (III) | Rideout et al., 2010 | A gift from Stephen Goodwin |
|
| Genetic reagent (D. melanogaster) |
Dsx-LexA::P65 (III) | Zhou et al., 2015 | A gift from Bruce Baker |
|
| Genetic reagent (D. melanogaster) |
UAS-2xEGFP (II) | BDSC: 6874; RRID:BDSC_6874 |
||
| Genetic reagent (D. melanogaster) |
R71G01-LexA (II) | Pan et al., 2012 | BDSC: 54733; RRID:BDSC_54733 |
|
| Genetic reagent (D. melanogaster) |
R41A01-LexA (II) | Zhou et al., 2014 | BDSC: 54787; RRID:BDSC_54787 |
|
| Genetic reagent (D. melanogaster) |
8xLexAop2-FLP (II) | BDSC: 55820; RRID:BDSC_55820 |
||
| Genetic reagent (D. melanogaster) |
UAS > STOP > TNT (III) | Stockinger et al., 2005 | A gift from Barry Dickson |
|
| Genetic reagent (D. melanogaster) |
UAS-ReachR (III) | Inagaki et al., 2014 | BDSC: 53749; RRID:BDSC_53749 |
|
| Genetic reagent (D. melanogaster) |
VT25602.p65ADZp (II) | Wu et al., 2019 | A gift from Barry Dickson |
|
| Genetic reagent (D. melanogaster) |
VT2064.ZpGAL4DBD (III) | Wu et al., 2019 | A gift from Barry Dickson |
|
| Genetic reagent (D. melanogaster) |
UAS(FRT.mCherry) ReachR (III) |
Inagaki et al., 2014 | BDSC: 53740; RRID:BDSC_53740 |
A gift from David Anderson |
| Genetic reagent (D. melanogaster) |
R71G01-p65.AD (II) | Dionne et al., 2018 | BDSC: 70798; RRID:BDSC_70798 |
A gift from Gerry Rubin |
| Genetic reagent (D. melanogaster) |
Dsx.DBD (III) | Pavlou et al., 2016 | A gift from Stephen Goodwin |
|
| Genetic reagent (D. melanogaster) |
10xUAS-IVS-myr::GFP (II) | BDSC: 32198; RRID:BDSC_32198 |
||
| Genetic reagent (D. melanogaster) |
W+,NorpA (X) | BDSC: 9048; RRID:BDSC_9048 |
||
| Genetic reagent (D. melanogaster) |
VT25602-Gal4 (III) | A gift from Barry Dickson |
||
| Genetic reagent (D. melanogaster) |
R57C10-LexA (II) | BDSC: 52817; RRID:BDSC_52817 |
A gift from Gerry Rubin |
|
| Genetic reagent (D. melanogaster) |
8xLexAop- mCD8tdTomato (III) |
A gift from Yuh Nung Jan |
||
| Genetic reagent (D. melanogaster) |
13xLexAop-IVS- GCaMP6s (III) |
BDSC: 44274; RRID:BDSC_44274 |
||
| Genetic reagent (D. melanogaster) |
10xUAS-IVS-Syn21- Chrimson-tdT-3.1 (X) |
Hoopfer et al., 2015 | A gift from Allan Wong |
|
| Genetic reagent (D. melanogaster) |
13xLexAop2-IVS- Syn21-opGCaMP6s (X) |
A gift from Allan Wong | ||
| Genetic reagent (D. melanogaster) |
20xUAS-IVS- CsChrimson.mVenus (X) |
BDSC: 55134 RRID:BDSC_55134 |
A gift from Vivek Jayaraman |
|
| Genetic reagent (D. melanogaster) |
Fru[P1.LexA] (III) | BDSC: 66698 RRID:BDSC_66698 |
A gift from Bruce Baker | |
| Antibody | Mouse anti-brp | mAb DSHS - developmental studies Hybridoma bank |
#nc82; RRID:AB_2314866 |
(1:20 dilution) |
| Antibody | Chicken anti-GFP (Polyclonal) |
Invitrogen | #A10262, RRID:AB_2534023 |
(1:2000 dilution) |
| Antibody | Rabbit anti-dsRed (Polyclonal) |
Takara Bio | #632496, RRID:AB_10013483 |
(1:500 dilution) |
| Antibody | Goat anti-mouse Alexa-Flour 568 (Polyclonal) |
Invitrogen | #A-11004, RRID:AB_2534072 |
(1:400 dilution) |
| Antibody | Goat anti-chicken Alexa-Flour 488 (Polyclonal) |
Invitrogen | #A11039, RRID:AB_142924 |
(1:300 dilution) |
| Antibody | Goat anti-rabbit Alexa-Flour 633 (Polyclonal) |
Invitrogen | #A-21070, RRID:AB_2535731 |
(1:400 dilution) |
| Chemical compound, drug | All trans-Retinal | Sigma-Aldrich | #R2500 | |
| Software, algorithm | MATLAB | Mathworks | RRID:SCR_001622 | |
| Software, algorithm | SLEAP | https://sleap.ai/ | ||
| Software, algorithm | Illustrator | Adobe | RRID:SCR_010279 |