Figure 3. An unbiased screen suggests that aIPg1-3 and pC1d are key nodes for gating female-female aggression.

(A) The fourteen top hits for female-female chasing from an unbiased activation screen of 2204 generation 1 GAL4 lines are listed along with their Z-scores, the signed number of standard deviations from the mean behavior of the control, as determined by Robie et al., 2017. Also shown is the relevant cell type we concluded from our intersectional analysis (Figure 4—figure supplement 1) to be present in each line. N.D., no cell type reproducibly detected, suggesting that R48F12 may not share a common cell type with any of the other 13 lines. The final column in the table refers to the panels in Figure 3—figure supplement 1 where results supporting the stated conclusion are shown. (B,C) The expression patterns of the two indicated GAL4 lines (Jenett et al., 2012). The images shown were taken from the database at http://www.janelia.org/gal4-gen1, where the expression patterns of the other lines listed in A can also be found. (D) The expression pattern of a split-GAL4 line made by intersecting these two enhancers; a cell with the morphology of pC1d can be seen.

Figure 3—figure supplement 1. Expression patterns resulting from split-GAL4 intersections of hits from the unbiased screen.

(A–I) Each panel shows the intersection of the indicated enhancers; the enhancer in the lower left was used to drive the split-GAL4 AD domain and the enhancer in the lower right drove the DBD domain. We observed either the pC1d or aIPg cell type in each intersection. Nearly all of the pC1d containing intersections also show expression in pC1e or other members of the pC1 cell-type group.