Figure 9. Absence of visual information alters aggressive behaviors during aIPgSS1 stimulation.

(A) Percentage of individual female flies engaging in aggressive behaviors over the course of a 2 min trial during which a 30 s 0.1 mW/mm² continuous light stimulus was delivered (pink shading). Trials were conducted either in the presence or absence of visible light, as indicated. (B) Fraction of time individuals spent performing aggressive behaviors during the stimulus period. Each dot represents the mean of the individuals within each experiment. (C) Ethogram plots of the aggressive events in light (white) and dark (gray) conditions. Each row represents one individual in a trial and black bars represent classified aggressive events. The stimulus period is indicated in pink and the ethograms were chosen to represent a typical trial for each condition. (D) Time course of example aggressive events during a single bout in light (top) and dark (bottom) conditions. The trajectory of one fly is shown both prior to (blue), during (orange), and immediately following (blue) an aggressive bout. The time before (-) and after (+) a central frame of the behavior is shown in the upper left corner of each image. Note that although average bout length in longer in the light, we selected bouts of similar length for display to facilitate comparison. The scale bar (3 mm) applies throughout each condition. See Video 15 for examples of multiple bouts in the light and dark. (E) Mean length of classified aggressive behavioral events during the stimulus period. Event lengths were calculated from the per-frame classifications. Each dot represents the mean of the individuals within each experiment. (F) Polar plots of the angle on the targeted fly (θ), measured as shown in the diagram at the left, during classified aggressive events. Each wedge represents 15 degrees and plots were normalized within each condition. Light: EmptySS > Chrimson, n = 89 flies in eight experiments; aIPgSS1 > Chrimson, n = 136 flies in eight experiments; Dark: EmptySS > Chrimson, n = 90 flies in eight experiments; aIPgSS1 > Chrimson, n = 126 flies in eight experiments. Box-and-whisker plots in panels B and E show median and IQR; whiskers show range. A Mann-Whitney U test was used for statistical analysis. Asterisk indicates significance: **p<0.01.

Figure 9—figure supplement 1. Connections between visual projection neurons, interneurons, and aIPg type 1, type 2 and type 3.

Connections with 30 or more combined synapses for each population of visual projection neurons to interneurons are shown. Inputs to the aIPg neurons were thresholded in proportion to the number of cells in each aIPg type resulting in thresholds of 24, 30, and 12 synapses to type 1, type 2, and type 3, respectively. The color-coding of the circles representing interneurons indicates the fraction of their outputs going to each aIPg type.